Imugex

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Categories: Biochemicals / Biochemicals

Product name			Second-Strand cDNA Synthesis Kit-dNTP/dUTP based
Catalog No			IGX-087
Supplier’s Catalog No			IGX-087
Supplier			ImuGeX
Source antigen
Reactivity
Cross reactivity
Applications
Molecular weight

Storage			Store all components at -20°C. All components are stable for 1 year from the date of shipping when stored and handled properly. Avoid repeated freeze-thaw cycles to retain maximum performance.
Other names
Grade
Purity
Form
Reactivity life			12 months
Note			For reserch purpose only
Description			Second Strand cDNA Synthesis Kit is an efficient system of generating double stranded cDNA from first strand cDNA as a template. The E. coli RNase H nicks the RNA in the DNA:RNA hybrid, while the E. coli DNA Polymerase replaces the RNA with deoxyribonucleotides. The E. coli DNA Ligase completes the double stranded DNA formation by linking the gaps between the newly synthesized cDNA strand. The dNTP based kit (Cat. No. IGX-086) and dNTP/dUTP based kit (Cat. No. IGX-087) provides different combination of deoxyribonucleotides tailored to the end user's needs and application. This kit is provided as individual enzymes to meet the customer's needs and to provide maximum efficiency and flexibility in the RNA sample preparation. (Noted that these enzymes may still contain E. coli gDNA) The double stranded cDNA end product can subsequently be converted to blunt ended DNA fragments using ImuGeX's DNA End Repair Kit (Cat. No. G477), followed by ImuGeX's dA Tailing Kit (Cat. No. E009) to generate DNA suitable for whole genome sequencing.