Apolipoprotein E (ApoE, AD2, MGC1571, Apoprotein, Apolipoprotein E, Apolipoprotein E3, Alzheimer Disease 2 (APOE*E4-associated, late onset) (Control Peptide)
Catalog No : USB-A2299-75E-P
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| Product name | Apolipoprotein E (ApoE, AD2, MGC1571, Apoprotein, Apolipoprotein E, Apolipoprotein E3, Alzheimer Disease 2 (APOE*E4-associated, late onset) (Control Peptide) | ||
|---|---|---|---|
| Catalog No | USB-A2299-75E-P | ||
| Supplier’s Catalog No | A2299-75E-P | ||
| Supplier | US Biologicals | ||
| Source antigen | Synthetic | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | Highly Purified | ||
| Form | Supplied as a lyophilized powder in PBS. Reconstitution: Reconstitute with 200ul sterile ddH2O. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Description | C-VGTSAAPVPSDNH from the C-Terminus of human APOE. Control peptide corresponding to polyclonal antibody, Catalog#A2299-75E, goat x human. Apolipoprotein E serves as a ligand for low density receptors and participates in the transport and redistribution of cholesterol and other lipids. Other functions include immunoregulation and cell growth modulation and differentiation. Apo E is thought to be involved in tissue repair as increase amounts of the protein are found at sites or peripheral nerve injury and regeneration. A mutant form is associated with familial type hyperlipoproteinemia. Applications: Suitable for use in ELISA. Other Applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher Positive Control: Human Brain and liver cells Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 monthsat -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking (see corresponding antibody A2299-75E): Antibodies are typically supplied at 0.5mg/ml and peptides as a 100ul pellet. When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel. | ||
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