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Categories: Biochemicals / Biochemicals

Product name			Apolipoprotein A-I (Apo A-I)
Catalog No			USB-A2299-30
Supplier’s Catalog No			A2299-30
Supplier			US Biologicals
Source antigen			High-density lipoprotein (HDL) from pooled human plasma
Reactivity
Cross reactivity
Applications
Molecular weight

Storage			-20°C
Other names
Grade			Highly Purified
Purity			>99% (SDS-PAGE). Isolated by ultracentrifugal flotation between densities 1.063–1.21g/mLl lyophilized, then subjected to repeated ethanol extraction. The precipitated protein was dried under nitrogen then dissolved in 6M guanidine hydrochloride and ~25mM DTT. Product was then subjected to Sephacryl S200 filtration and eluted.
Form			Supplied as a liquid containing 3M guanidine hydrochloride, 10mM Tris, 100mM sodium chloride, 1mM EDTA and 1mM sodium azide, pH 7.4.
Reactivity life			12 months
Note			For reserch purpose only
Description			Preparation of Protein: Apo AI can be renatured into PBS, TBS, or other common buffers by dialysis using a membrane with a nominal molecular weight cut-off of 14,000 or less. This should be performed in a cold room. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Dialyzed Apo AI in PBS or TBS with 1mM EDTA and 0.1% sodium azide may be stored for up to 2 weeks at 4°C. Storage of Apo AI in PBS or TBS at -20°C may lead to precipitation when thawed.