Imugex

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Categories: Biochemicals / Biochemicals

Product name			ImmunoTek Plus™ Yellow Stop Reagent, Liquid (450nm)
Catalog No			USB-172016
Supplier’s Catalog No			172016
Supplier			US Biologicals
Source antigen
Reactivity
Cross reactivity
Applications
Molecular weight

Storage			4°C
Other names
Grade
Purity
Form
Reactivity life			12 months
Note			For reserch purpose only
Description			ImmunoTek Plus™ Yellow Stop Reagent, Liquid (450nm) This is a proprietary liquid reagent used to stop the one-component TMB and other TMB formulation (3,3’,5,5’-tetramethylbenzidine) reactions by changing the chromogen into the second oxidation state of TMB and form a yellow reaction product read ~450nm. Product Use: This product is used to terminate TMB Microwell substrates and change the absorbance from blue (650nm) to yellow (450nm). Equal volumes of substrate and terminator solution should be used. When TMB peroxidase substrate solution (3,3’,5,5’-tetramethylbenzidine) is reacted with peroxidase, a reaction ensues producing a blue colored liquid product (oxidized state). The reaction can be terminated using proprietary Yellow Stop specifically designed for TMB substrates. Following termination of the reaction, a second oxidized state is produced resulting in a soluble yellow reaction product. The colored product is stable for at least two hours. Storage and Stability: Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.