XRCC6 (X Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6, X-ray Repair Complementing Defective Repair in Chinese Hamster Cells 6, 70kD Subunit of Ku Antigen, ATP Dependent DNA Helicase 2 Subunit 1, ATP-dependent DNA Helicase II 70kD Subunit, CTC Box Binding Factor 75kD Subunit, CTC75, CTCBF, DNA Repair Protein XRCC6, G22P1, Ku 70, Ku70, Ku-70, Ku Autoantigen 70kDa, Lupus Ku Autoantigen Protein p70, ML8, Thyroid Lupus Autoantigen, TLAA) (Control Peptide)
Catalog No : USB-X9506-05J-P
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| Product name | XRCC6 (X Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6, X-ray Repair Complementing Defective Repair in Chinese Hamster Cells 6, 70kD Subunit of Ku Antigen, ATP Dependent DNA Helicase 2 Subunit 1, ATP-dependent DNA Helicase II 70kD Subunit, CTC Box Binding Factor 75kD Subunit, CTC75, CTCBF, DNA Repair Protein XRCC6, G22P1, Ku 70, Ku70, Ku-70, Ku Autoantigen 70kDa, Lupus Ku Autoantigen Protein p70, ML8, Thyroid Lupus Autoantigen, TLAA) (Control Peptide) | ||
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| Catalog No | USB-X9506-05J-P | ||
| Supplier’s Catalog No | X9506-05J-P | ||
| Supplier | US Biologicals | ||
| Source antigen | Synthetic | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | Highly Purified | ||
| Form | Supplied as a lyophilized powder in PBS. Reconstitution: Reconstitute with 200ul sterile ddH2O. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | Highly Purified | ||
| Description | YKTEGDEEAEEEQ-C, from the N-Terminal of human XRCC6. Control peptide corresponding to polyclonal antibody, Catalog#X9506-05J, goat x human. Originally identified as an autoantigen, the Ku proteins are nuclear proteins found in eukaryotes. They exist as a heterodimer consisting of Ku70 and Ku80, and migrate at approximately 70 and 80kD, respectively. They bind to DNA ends at double-strand breaks. The Ku proteins interact with the DNA-dependent protein kinase (DNA-PK), and participate in the targeting of DNA-PK to DNA. The Ku proteins may participate in numerous cellular processes, including DNA repair and replication, cell signaling, and proliferation, transcriptional activation, and apoptosis. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 monthsat -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking (see corresponding antibody X9506-05J): Antibodies are typically supplied at 0.5mg/ml and peptides as a 100ul pellet. When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel. | ||
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