Hepcidin, 25aa, oxidized, Mouse, Control Peptide (Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed Antimicrobial Peptide) (Biotin)
Catalog No : USB-H2008-52B
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| Product name | Hepcidin, 25aa, oxidized, Mouse, Control Peptide (Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed Antimicrobial Peptide) (Biotin) | ||
|---|---|---|---|
| Catalog No | USB-H2008-52B | ||
| Supplier’s Catalog No | H2008-52B | ||
| Supplier | US Biologicals | ||
| Source antigen | Mouse synthetic peptide | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Purified | ||
| Purity | ≥ 95% by HPLC | ||
| Form | Supplied as a lyophilized powder. Labeled with Biotin. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | ≥ 95% by HPLC | ||
| Description | Hepcidin (Hepc, hepatic bactericidal protein) or LEAP (liver expressed antimicrobial peptide) is small, cysteine-rich peptide, antimicrobial peptide similar to defensins and thionins. Hepcidin (unprocessed, proprotein in mouse 83 aa, rat/human 84 aa) are almost exclusively produced in liver. Human hepcidin is produced from 84-aa precursor, including a putative 24-aa signal peptide. The secreted form of hepcidin in blood and urine is consists of C-terminal 20, 22 or 25-aa residues in humans and 25-aa in mouse. In humans, 20-aa and 25-aa appears to be the major HEPC secreted peptides with antimicrobial activities. The three secreted HEPC alternatively spliced HEPC peptides differ at the N-terminus. Human DTHFP ICIFC CGCCH RSKCG MCCKT (25-aa) ICIFC CGCCH RSKCG MCCKT (20-aa) Mouse DTNFP ICIFC CKCCN NSQCG ICCKT (25 aa) 4 cys-Cys bridges The link between hepcidins and iron metabolism is that hepcidin expression is abolished in mice exhibiting iron-overload due to the targeted disruption of USF2 (upstream stimulatory factor 2) gene resembling the situation in hfe-/- mice. The human gene is located at chromosome 19, in close proximity with Usf2 gene. Hepcidin levels are increased in iron loading and in beta-2 microglobulin knockout mice. Hepcidins are devoid of IRE. Like other antimicrobial peptides, hepcidin is up-regulated by lipopolysaccharides (LPS). Source: Human and mouse HEPC (20 and 25-aa, sequence given above) were synthesized. Cysteines were air-oxidized assuming that they will assume natural cys-cys bridging. We do not know the order of Cys-Cys bridges. Mol. Wt: 2763-human HEPC 25 aa Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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