Abl Kinase Substrate (Biotin) (Abltide)

Catalog No : USB-A0007-05
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Product name Abl Kinase Substrate (Biotin) (Abltide)
Catalog No USB-A0007-05
Supplier’s Catalog No A0007-05
Supplier US Biologicals
Source antigen
Reactivity
Cross reactivity
Applications
Molecular weight 1563
Storage -20°C
Other names
Grade Highly Purified
Purity ~ 90%, as determined by HPLC
Form Supplied as a lyophilized powder.
Reactivity life 12 months
Note For reserch purpose only
Purity ~ 90%, as determined by HPLC
Description Synthetic (linear) peptide (EAIYAAPFAKKK), biotinylated at the C-terminus. Substrate for Abl kinase. (with an extra K conjugated to Biotin) Application: Suitable for use in Abl kinase assays. Other applications not tested. Recommended Dilution: Use at 25-50uM final concentration for in vitro Abl kinase assays. 25uM was phosphorylated using 10-20ng of Abl, active. The substrate was captured using Streptavidin, agarose conjugate. Assay results show a 332-fold increase in phosphate transferred to the peptide over background. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Stable for 12 months at -20°C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20°C.