IgG Fab, Rabbit (Biotin)

Catalog No : USB-I1904-38H
450.59€
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Product name IgG Fab, Rabbit (Biotin)
Catalog No USB-I1904-38H
Supplier’s Catalog No I1904-38H
Supplier US Biologicals
Source antigen Rabbit
Reactivity IEP Assay shows a single precipitin arc against anti-biotin, anti-rabbit IgG, anti-rabbit IgG F(ab’)2 and anti-rabbit serum. No reaction observed against anti-rabbit IgG F(c) or anti-Pepsin.
Cross reactivity
Applications
Molecular weight
Storage -20°C
Other names
Grade Highly Purified
Purity Purified by delipidation, salt fractionation and ion exchange chromatography, followed by pepsin digestion and dialysis.
Form Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), and 0.01% sodium azide. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Reactivity life 12 months
Note For reserch purpose only
Description Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG Fab molecule Applications: Suitable for use in Western Blot, ELISA, Immunohistochemistry and immunomicroscopy. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.