Hepcidin, Mouse, 13aa, CT, Control Peptide (Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed Antimicrobial Peptide)
Catalog No : USB-H2008-55
417.25€
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| Product name | Hepcidin, Mouse, 13aa, CT, Control Peptide (Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed Antimicrobial Peptide) | ||
|---|---|---|---|
| Catalog No | USB-H2008-55 | ||
| Supplier’s Catalog No | H2008-55 | ||
| Supplier | US Biologicals | ||
| Source antigen | Mouse synthetic peptide | ||
| Reactivity | Synthetic peptide 13aa sequence within the C-terminus of mature, 25aa mouse HEPC. Species Sequence Homology: Rat: 92%; Human: 69% (20-, 22- and 25-aa C-terminal fragments). | ||
| Cross reactivity | Mouse, | ||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | Highly purified | ||
| Form | Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | Highly purified | ||
| Description | Control Peptide for H2008-53 (antiserum) and H2008-54 (affinity purified antibody). Hepcidin (Hepc, hepatic bactericidal protein) or LEAP (liver expressed antimicrobial peptide) is small, cysteine-rich peptide, antimicrobial peptide similar to defensins and thionins. Hepcidin (unprocessed, proprotein in mouse 83 aa, rat/human 84 aa) are almost exclusively produced in liver. Human hepcidin is produced from 84-aa precursor, including a putative 24-aa signal peptide. The secreted form of hepcidin in blood and urine is consists of C-terminal 20, 22 or 25-aa residues. The link between hepcidins and iron metabolism is that hepcidin expression is abolished in mice exhibiting iron-overload due to the targeted disruption of USF2 (upstream stimulatory factor 2) gene resembling the situation in hfe-/- mice. The human gene is located at chromosome 19, in close proximity with Usf2 gene. Hepcidin levels are increased in iron loading and in beta-2 microglobulin knockout mice. Hepcidins are devoid of IRE. Like other antimicrobial peptides, hepcidin is upregulated by lipopolysaccharides (LPS). Applications: Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested. Recommended Dilution: ELISA: 50-100ng control peptide/well. Antibody Blocking: 5-10ug per 1ul H2008-53 (antiserum) or per 1ug H2008-54 (affinity purified antibody). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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