Osteoprotegerin/Fc Chimera, Recombinant, Human (OPG-Fc)

Catalog No : USB-360925
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Product name Osteoprotegerin/Fc Chimera, Recombinant, Human (OPG-Fc)
Catalog No USB-360925
Supplier’s Catalog No 360925
Supplier US Biologicals
Source antigen Pichia Pastoris
Reactivity
Cross reactivity
Applications
Molecular weight 46.5
Storage -20°C
Other names
Grade Highly Purified
Purity ~90% (SDS-PAGE, HPLC)
Form Supplied as a lyophilized powder in PBS, pH 7.4. Reconstitute with sterile dH2O, 0.1% BSA to 0.1-1mg/ml.
Reactivity life 6 months
Note For reserch purpose only
Purity ~90% (SDS-PAGE, HPLC)
Description Source: Recombinant protein corresponding to 412aa residues, including 180 residues from mature OPG (aa22-201) and 232 residues from the Fc protein of human IgG1 from human Osteoprotegerin/Fc Chimera expressed in Pichia Pastoris. Molecular Weight: ~46.5kD (412aa) Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Biological Activity: Fully biologically active when compared to the standard. Determined by its ability to neutralize the stimulation of U937 cells treated with 10ng/ml of soluble RANKL. Endotoxin: <1EU/mg (LAL) AA Sequence: ETFPPKYLHYDEETSHQLLCDKCPPGTYLKQHCTAKWKTVCAPCPDHYYTDSWHTSDECLYCSPVCKELQYVKQECNRTHNRVCECKEGRYLEIEFCLKHRSCPPGFGVVQGTPERNTVCKRCPDGFFSNETSSKAPCRKHTNCSVFGLLLTQKGNATHDNICSGN SESTQKCGIDVTL Fc232: EPKSSDKTHTCPPCPAPEFEGAPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPTPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile dH2O, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.