Nampt, Recombinant, Human (Visfatin/PBEF, PBEF1, Pre-B Cell Colony Enhancing Factor 1, Nicotinamide Phosphoribosyltransferase)
Catalog No : USB-045069
459.78€
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| Product name | Nampt, Recombinant, Human (Visfatin/PBEF, PBEF1, Pre-B Cell Colony Enhancing Factor 1, Nicotinamide Phosphoribosyltransferase) | ||
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| Catalog No | USB-045069 | ||
| Supplier’s Catalog No | 045069 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, Human, HEK 293 cells | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 57100 | ||
| Storage | 4°C/-20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | ≥95% (SDS-PAGE). Endotoxin: <0.01EU/ug (LAL test; Lonza). | ||
| Form | Supplied as a lyophilized powder from a 0.2um-filtered solution in PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ≥95% (SDS-PAGE). Endotoxin: <0.01EU/ug (LAL test; Lonza). | ||
| Description | Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kD adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. Biological Activity: Shows adipogenic effects in stimulated differentiating 3T3-L1 cells. Sequence: Human Nampt (visfatin/PBEF) (aa1-491) is fused at the N-terminus to a FLAG®-tag. Molecular Weight: ~57kD (SDS-PAGE); ~100kD (Dimer) (SEC) Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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