PBEF, Recombinant, Human (Pre-B cell Colony Enhancing Factor, Pre-B cell Enhancing Factor, 1110035O14Rik, DKFZP666B131, MGC117256, Nicotinamide Phosphoribosyltransferase, NAmPRTase, NAMPT, Pre-B cell Colony Enhancing Factor 1, PBEF1, Visfatin, VF)
Catalog No : USB-P3113-97R2
402.31€
0.00€
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| Product name | PBEF, Recombinant, Human (Pre-B cell Colony Enhancing Factor, Pre-B cell Enhancing Factor, 1110035O14Rik, DKFZP666B131, MGC117256, Nicotinamide Phosphoribosyltransferase, NAmPRTase, NAMPT, Pre-B cell Colony Enhancing Factor 1, PBEF1, Visfatin, VF) | ||
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| Catalog No | USB-P3113-97R2 | ||
| Supplier’s Catalog No | P3113-97R2 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 55 | ||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~95% (SDS-PAGE). Endotoxin: ≤0.1ng/ug. | ||
| Form | Supplied as a lyophilized powder with no additives. Reconstitute with sterile PBS to 0.1-1mg/ml. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ~95% (SDS-PAGE). Endotoxin: ≤0.1ng/ug. | ||
| Description | Visfatin is a putative cytokine originally identified as PBEF (Pre-B cell-enhancing factor), subsequently determined to be a nicotinamide phosphoribosyltransferase (NAMPT) enzyme and member of the NAPRTase family, involved in catalyzing the biosynthesis of NAD from nicotinamide. Visfatin is highly expressed in bone marrow, muscle and liver tissue and increases in expression in response to endotoxin, in amniotic epithelial cells following lipopolysaccharide (LPS) stimulation, and during the activation of T and B cells, dendritic cells and macrophages, suggesting a role in inflammatory processes. Recombinant corersponding to full length, human visfatin (a.a.1-491) fused at the N-terminus to a His-Tag, expressed in E. coli. Biological Activity: Mimics insulin effects in differentiating 3T3-L1 cells. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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