Acrp30, Recombinant, Mouse (Adipocyte Complement-related Protein of 30kD, Adiponectin, AdipoQ, apM1, Gelatin Binding Protein 28, GPB28)

Catalog No : USB-A0725-03L
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Product name Acrp30, Recombinant, Mouse (Adipocyte Complement-related Protein of 30kD, Adiponectin, AdipoQ, apM1, Gelatin Binding Protein 28, GPB28)
Catalog No USB-A0725-03L
Supplier’s Catalog No A0725-03L
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight
Storage -20°C
Other names
Grade Highly Purified
Purity ≥ 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Form Supplied as a liquid in 20mM Tris-HCl pH7.5, 50mM NaCl, 5mM DTT and 10% Glycerol.
Reactivity life 6 months
Note For reserch purpose only
Purity ≥ 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Description Adiponectin/Acrp30 (247amino acids) is adipocyte complement-related protein of 30kD and exclusively expressed in differentiated adipocytes. Adiponectin(Acrp30) is a member of the complement factor C1q family and consists of signal sequence, Non-homologous sequence, collagen domain and globular domain(gAcrp30). Adiponectin(Acrp30) expression is reduced in a variety of obese and insulin-resistant states in human, monkeys and mice. Injection of Acrp30(247aa) or gAcrp30(globular domain) lowers serum glucose and free fatty acid level in mice. The globular domain of adiponectin/acrp30(amino acid residues, 111-247, gAcrp30) was overexpressed in E.coli and purified by using conventional chromatography techniques. Sequence: MAYMYRSAFS VGLETRVTVP NVPIRFTKIF YNQQNHYDGS TGKFYCNIPG LYYFSYHITVYMKDVKVSLF KKDKAVLFTY DQYQEKNVDQ ASGSVLLHLE VGDQVWLQVY GDGDHNGLYADNVNDSTFTG FLLYHDTN Endotoxin: ≤ 0.1ng/ug (IEU/ug) of Recombinant Acrp30. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.