Imugex

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Categories: Biochemicals / Biochemicals

Product name			Ubiquitin-AMC Substrate, Fluorogenic (Ubiquitin-aminomethylcoumarin)
Catalog No			USB-U0999-07
Supplier’s Catalog No			U0999-07
Supplier			US Biologicals
Source antigen
Reactivity
Cross reactivity
Applications
Molecular weight			8.6

Storage			-70°C
Other names
Grade			Purified
Purity			~95% (HPLC)
Form			Supplied as a liquid in DMSO.
Reactivity life			12 months
Note			For reserch purpose only
Description			Prepared by the C-terminal derivatisation of ubiquitin with 7-amino-4-methylcoumarin. Acts as a useful and sensitive fluorogenic substrate for a wide range of deubiquitinylating enzymes (DUBs), including ubiquitin C-terminal hydrolases (UCHs) and ubiquitin specific proteases (USPs). Ubiquitin-AMC has been shown to be a sensitive substrate for UCH-L3 (Km=0.039uM) and for Isopeptidase-T (Km=0.17-1.4uM), as well as for studying deubiquitinylating activity where detection sensitivity or continuous monitoring of activity is essential. Sequence: C-terminal derivatisation of ubiquitin with 7-amino-4-methylcoumarin Emission: 460nm Excitement: 380nm Molecular Weight: ~8.6kD Substrate Concentration: 0.01-1uM Enzyme Concentration: 10-100pM (UCH-L3); 10-100nM (Isopeptidase-T) Applications: Suitable for use in Deubiquitinylating Enzyme Activity. Other applications not tested. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.