Matrix Metalloproteinase 9 (MMP9) Recombinant, Mouse

Catalog No : USB-155838
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Product name Matrix Metalloproteinase 9 (MMP9) Recombinant, Mouse
Catalog No USB-155838
Supplier’s Catalog No 155838
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 19.9
Storage -70°C/-20°C
Other names
Grade Highly Purified
Purity ≥95%. Endotoxin: ≤1EU/ug (LAL)
Form Supplied as powder lyophilized from 20mM Tris, 150mM sodium chloride, pH 8.0, 1mM EDTA, 1mM DTT, 5% trehalose,, 0.01% sarcosyl, 0.01% sodium azide. Reconstitute with sterile ddH2O.
Reactivity life 6 months
Note For reserch purpose only
Purity ≥95%. Endotoxin: ≤1EU/ug (LAL)
Description Recombinant protein corresponding to aa225-390 with an N-Terminal His-Tag from mouse MMP9 expressed in E. coli (P41245) Amino Acid Sequence: MGHHHHHHSG SEF-SNGAPC HFPFTFEGRS YSACTTDGRN DGTPWCSTTA DYDKDGKFGF CPSERLYTEH GNGEGKPCVF PFIFEGRSYS ACTTKGRSDG YRWCATTANY DQDKLYGFCP TRVDATVVGG NSAGELCVFP FVFLGKQYSS CTSDGRRDGR LWCATTSNFD TDKKWGFCPD Predicted Molecular Weight: 19.9kD Predicted Isoelectric Point: 6.2 Subcellular Location: Secreted, extracellular space, extracellular matrix Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Included Protein Molecular Weight Marker: 168631: Unstained Protein Molecular Weight Marker, 10-70kD. No heating, diluting or reducing agents needed. MW: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD, 70kD Double intensity: 10kD, 18kD, 26kD Supplied in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Add 3-5ul/well for mini gels; 7ul/well for large gels. Store at -20°C Stable for 6 months after receipt. Storage and Stability: Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.