Matrix Metalloproteinase 13 (MMP13) Recombinant, Mouse

Catalog No : USB-155821
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Product name Matrix Metalloproteinase 13 (MMP13) Recombinant, Mouse
Catalog No USB-155821
Supplier’s Catalog No 155821
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 21
Storage -70°C
Other names
Grade Highly Purified
Purity ≥95% Endotoxin: ≤1EU/ug (LAL)
Form Supplied as lyophilized powder from PBS, pH 7.4, 1mM DTT, 5% Trehelose, 0.01% sarcosyl. Reconstitute in sterile PBS, pH 7.3.
Reactivity life 12 months
Note For reserch purpose only
Purity ≥95% Endotoxin: ≤1EU/ug (LAL)
Description Source: Recombinant protein corresponding to aa104-291 from mouse Matrix Metalloproteinase 13, expressed in E. coli with N-terminal His-tag (Accession# P33435). Sequence: MGHHHHHHSGSEF- VDRLR EMQSFFGLEV TGKLDDPTLD IMRKPRCGVP DVGEYNVFPR TLKWSQTNLT YRIVNYTPDM SHSEVEKAFR KAFKVWSDVT PLNFTRIYDG TADIMISFGT KEHGDFYPFD GPSGLLAHAF PPGPNYGGDA HFDDDETWTS SSKGYNLFIV AAHELGHSLG LDHSKDPG Preditcted Molecular Weight: 22.6kD Subcellular Location: Secreted, extracellular Predicted isoelectric point: 5.6 Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -80°C. Aliquots are stable for at least 12 months from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Included Protein Molecular Weight Marker: 168631: Protein Molecular Weight Marker, Unstained Ready to use, No heating, diluting or reducing agents needed. MW: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD, 70kD Double intensity: 10kD, 18kD, 26kD Supplied in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Add 3-5ul/well for mini gels; 7ul/well for large gels. Store at -20°C Stable for 6 months after receipt. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.