Matrix Metalloproteinase 11 (MMP11) Recombinant, Mouse
Catalog No : USB-155810
413.80€
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| Product name | Matrix Metalloproteinase 11 (MMP11) Recombinant, Mouse | ||
|---|---|---|---|
| Catalog No | USB-155810 | ||
| Supplier’s Catalog No | 155810 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 24.7 | ||
| Storage | -70°C/-20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ≥95%. Endotoxin: ≤1EU/ug (LAL) | ||
| Form | Supplied as lyophilized powder from PBS, pH 7.4, 1mM DTT, 0.05% sarcosyl, 5% trehalose, 0.09% sodium azide. Reconstitute in sterile PBS, pH 7.2. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | ≥95%. Endotoxin: ≤1EU/ug (LAL) | ||
| Description | Recombinant protein corresponding to aa287-491 with an N-Terminal His-Tag from mouse MMP11, expressed in E. coli (Q7TPW7) Sequence: MGHHHHHHSG SEF-ALLEPET PPDVCETSFD AVSTIRGELF FFKAGFVWRL RSGRLQPGYP ALASRHWQGL PSPVDAAFED AQGQIWFFQG AQYWVYDGEK PVLGPAPLSK LGLQGSPVHA ALVWGPEKNK IYFFRGGDYW RFHPRTQRVD NPVPRRSTDW RGVPSEIDAA FQDAEGYAYF LRGHLYWKFD PVKVKVLEGF PRPVGPDFFD CAEPANTF Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Accurate Molecular Mass: 17kD as determined by SDS-PAGE reducing conditions Note: The possible reasons that the actual band size differs from the predicted are as follows: 1. Splice variants: Alternative splicing may create different sized proteins from the same gene 2. Relative charge: The composition of amino acids may affect the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins and then cleaved to give the active form. Included Protein Molecular Weight Marker: 168631: Unstained Protein Molecular Weight Marker, 10-70kD Suppled as a liquid in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Ready to use; no need to heat, dilute or add reducing agents before use. Protein Bands: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD and 70kD. Double Intesity Bands: The 26kD, 18kD and 10kD bands are at double intensity to make location and size approximation of proteins easier. Usage: Allow the marker to reach room temperature and mix thoroughly before use to ensure that any solids that may have precipitated at -20°C will go back into solution. Do not boil! Load the following volumes on SDS-PAGE gel: Mini gels: 3-5ul/well Large gel: 7ul/well The loading volumes listed above are recommended for gels with thickness of 0.75-1mm. The loading volume should be doubled for 1.5mm thick gels. Store at -20°C Stable for 6 months after receipt. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions. | ||
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