ADAM 12S, Recombinant, Human, Western Blot Positive Control (Disintegrin and Metalloproteinase Domain-containing Protein 12, ADAM 12, Short Form, Meltrin-alpha,UNQ346/PRO545 MLTN)

Catalog No : USB-A0859-26B
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Product name ADAM 12S, Recombinant, Human, Western Blot Positive Control (Disintegrin and Metalloproteinase Domain-containing Protein 12, ADAM 12, Short Form, Meltrin-alpha,UNQ346/PRO545 MLTN)
Catalog No USB-A0859-26B
Supplier’s Catalog No A0859-26B
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight
Storage -20°C
Other names
Grade Purified
Purity Purified (SDS-PAGE)
Form Supplied as a liquid in 25mM Tris-HCl, pH6.8, 50mM DTT, 1% SDS, 0.1% Bromophenol Blue, 2.5% glycerol.
Reactivity life 6 months
Note For reserch purpose only
Purity Purified (SDS-PAGE)
Description ADAM proteins are a family of membrane-anchored cell surface protein containing both a disintegrin and a metalloprotease domain. Their exact functions have not been fully characterised however, they are thought to mediate a number of cellular processes including differentiation, cell fusion, cell-cell interactions and shedding of plasma membrane bound growth factor precursors. Two forms of ADAM12 have been described: ADAM12S and ADAM12L (short and long forms). The short form (ADAM12S) is a soluble form, which lacks the transmembrane and cytoplasmic domains. ADAM12S expression is thought to be limited to the placenta, embryo and foetus although levels have been detected in some tumor cell lines. Source: Recombinant corresponding to aa208-738 from human ADAM12S, fused to N-terminal 6x His-tag, expressed in E. coli. Applications: Suitable for use in Western Blot as a control. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.