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Categories: Peptides & Proteins / Proteins

Product name			Matrix Metalloproteinase- 9 (MMP-9), Recombinant, Human, His-Tag
Catalog No			USB-M2425-41A
Supplier’s Catalog No			M2425-41A
Supplier			US Biologicals
Source antigen			Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight

Storage			-20°C
Other names
Grade			Highly Purified
Purity			~95% (SDS-PAGE)
Form			Supplied as a liquid.
Reactivity life			6 months
Note			For reserch purpose only
Purity			~95% (SDS-PAGE)
Description			Recombinant human MMP-9 comprises a 338aa fragment (113-450) corresponding to the catalytic domain of the protein and is expressed in E. coli with an N-terminal 6xHis tag. Matrix metalloproteinases are a family of zinc and calcium- dependent endopeptidases, which degrade extracellular matrix proteins. MMP-9 is secreted as a 92kDa zymogen. Cleavage of pro-MMP-9 results in the active enzyme with a molecular weight of ~82kDa. MMP-9 has a gelatin-binding domain consisting of three fibronectin type II units, a catalytic domain containing the zinc-binding site, a proline-rich type V collagen-homologous domain and a hemopexin-like domain. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.