Matrix Metalloproteinase 13, Recombinant, Human (MMP-13, Collagenase 3)
Catalog No : USB-M2428-27D
439.09€
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| Product name | Matrix Metalloproteinase 13, Recombinant, Human (MMP-13, Collagenase 3) | ||
|---|---|---|---|
| Catalog No | USB-M2428-27D | ||
| Supplier’s Catalog No | M2428-27D | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 27 | ||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | > 90% (SDS-PAGE) | ||
| Form | Supplied as a liquid in 0.05M Tris-HCl, pH 7.5, 0.2M NaCl, 5mM CaCl2, 20uM ZnSO4 and 0.1% BSA. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | > 90% (SDS-PAGE) | ||
| Description | Latent recombinant human pro-collagenase (MMP-13) also called collagenase-3 truncated from C-terminal. Recombinant Human MMP-13 produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 27kD. Recombinant MMP-13 is purified by proprietary chromatographic techniques. Biological Activity: Recombinant MMP-13 activity is determined by the cleavage of fluorogenic peptide, 100 ng of enzyme activated with APMA will digest 80% fluorogenic peptide substrate (0.1ml of 20µM solution) at 35°C for 30 minutes, (10µg0 assays). Applications: Used as a standard for assaying MMP-13 or for screening inhibitors. Stability: Recombinant MMP-13 although stable at 140C for 1 week, should be stored desiccated below -180C. Please avoid freeze-thaw cycles. | ||
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