Matrix Metalloproteinase 9, Recombinant, Human (MMP-9, Collagenase 4, Gelatinase B)
Catalog No : USB-M2425-22B
728.76€
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| Product name | Matrix Metalloproteinase 9, Recombinant, Human (MMP-9, Collagenase 4, Gelatinase B) | ||
|---|---|---|---|
| Catalog No | USB-M2425-22B | ||
| Supplier’s Catalog No | M2425-22B | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, NSO cells. | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 76 | ||
| Storage | -70°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~95% by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: ≤ 0.1EU/ug. | ||
| Form | Supplied as a liquid in 50mM Tris-HCl, 150mM sodium chloride, 10mM calcium chloride, 0.05% Brij 35. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ~95% by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: ≤ 0.1EU/ug. | ||
| Description | EC 3.4.24.35 Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 maybe be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain. Source: Human Matrix Metalloproteinase 9, Ala20-Asp707 (Gln279Arg) (Accession #P14780) expressed in NSO cells. Molecular Weight: The recombinant protein (Ala20 Asp707(Gln279Arg)) has a predicted molecular mass of 76kD. By SDS-PAGE, the apparent molecular mass is 97kD, under reducing conditions. Specific Activity: ~1300 pmoles/min/ug. Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for up to 6 months. | ||
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