Matrix Metalloproteinase 7, Pro, Recombinant (ProMMP-7, Promatrilysin)
Catalog No : USB-M2423-12
348.29€
0.00€
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| Product name | Matrix Metalloproteinase 7, Pro, Recombinant (ProMMP-7, Promatrilysin) | ||
|---|---|---|---|
| Catalog No | USB-M2423-12 | ||
| Supplier’s Catalog No | M2423-12 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ≥ 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. | ||
| Form | Supplied as a liquid in 25mM Tris-HCl, pH 7.5, 150mM sodium chloride, 5mM calcium chloride, 0.01% Brij-35, 0.02% sodium azide. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ≥ 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. | ||
| Description | Matrix metalloproteinase-7 (MMP-7) also known as matrilysin and PUMP (EC 3.4.24.23) cleaves a number of substrates including collagen types IV and X, elastin, fibronectin, gelatin, laminin and proteoglycans. MMP-7 is closely related to the stromelysin family members but is encoded by a different gene. MMP-7 is the smallest of all the MMPs consisting of a pro-peptide domain and a catalytic domain. It lacks the hemopexin-like domain common to other members of the MMPs. MMP-7 is secreted as a 28kD proenzyme and can be activated in vitro by organomercurials and trypsin and in vivo by MMP-3 to a 18kD active MMP-7 enzyme. Once activated, MMP-7 can activate pro-MMP-1 and pro-MMP-9 but not pro-MMP-2. MMP-7 is widely expressed having been reported in elevated levels in cycling endometrium as well as in colorectal cancers and adenomas, hepatocellular carcinomas, rectal carcinomas, and approximately 50% of gliomas. Dimers and Aggregates: ≤ 1% as determined by silver-stained SDS-PAGE gel analysis. Biological Activity: The specific activity was found to be ~1400IU/mg. Unit Definition: One unit is defined as the digestion of 1ug Azocoll/min at 37°C. Endotoxin: ≤ 0.1ng/ug (IEU/ug) of Recombinant ProMMP-7 Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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