Psophocarpus tetragonolobus (PTL/PTA II)

Catalog No : USB-518705
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Product name Psophocarpus tetragonolobus (PTL/PTA II)
Catalog No USB-518705
Supplier’s Catalog No 518705
Supplier US Biologicals
Source antigen Winged bean
Reactivity
Cross reactivity
Applications
Molecular weight 54
Storage -20°C
Other names
Grade Affinity purified
Purity Affinity purified
Form Supplied as a lyophilized powder.
Reactivity life 2 years
Note For reserch purpose only
Purity Affinity purified
Description Psophocarpus tetragonolobus lectin (PTL II, psophocarpin B1, a storage protein with chymotrypsin inhibitory activity), is isolated from winged bean seeds and purified by fractionation. The seed contains two lectins in which PTL II is the acidic of the two. This lectin agglutinates trypsinized and desialized erythrocytes of all three blood group types (A, B, and O). Nonpolar interactions play an important role in stabilization of the lectin-sugar complex. Projection of alpha1,3 linked Gal/GalNAc leads to steric hindrance and prevents the binding of blood group A and B reactive sugars.The unique specificity of PTL II makes it to be useful in isolation and characterization of terminally monofucosylated H-reactive oligosaccharides from those that are difucosylated or internally fucosylated. Source: Winged bean Blood Specificity: O (-SA) Sugar Specificity: N-Acetylgalactosamine, Galactose Inhibitory Carbohydrate: N-Acetylgalactosamine Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.