Narcissus pseudonarcissus (NPL/NPA)

Catalog No : USB-518598
383.92€
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Product name Narcissus pseudonarcissus (NPL/NPA)
Catalog No USB-518598
Supplier’s Catalog No 518598
Supplier US Biologicals
Source antigen Lent lily
Reactivity
Cross reactivity
Applications
Molecular weight 59
Storage -20°C
Other names
Grade Affinity purified
Purity Affinity purified
Form Supplied as a lyophilized powder.
Reactivity life 2 years
Note For reserch purpose only
Purity Affinity purified
Description Narcissus pseudonarcissus lectin or agglutinin (NPL/NPA) is affinity purified composed of four subunits. It agglutinates rabbit erythrocytes but is non-reactive with human erythrocytes. NPA has an isoelectric point between pH 4.2 and pH 4.6 with a specific binding activity toward alpha-D-mannose, although studies have suggested that the purified lectin may have greater affinity for internal mannosyl residues than for nonreducing terminal units. A buffer washed column elutes the sugar alpha-methylmannoside. Human studies have found NPA to be nearly non-mitogenic, but it has been reported through an in vitro study that mitogenicity of NPA is age-related, with lymphoproliferative response of peripheral blood mononuclear cells (PBMC) in umbilical cord blood has a sevenfold increase than adult blood. NPA has also been found to be mitogenic for PBMC from lambs and not adult sheep. Source: Lent lily Blood Specificity: Rabbit Agglutinin Sugar Specificity: alpha-Mannose Inhibitory Carbohydrate: alpha-Methylmannoside Divalent Ions: None Required Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.