Narcissus pseudonarcissus (NPL/NPA)
Catalog No : USB-518598
383.92€
0.00€
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| Product name | Narcissus pseudonarcissus (NPL/NPA) | ||
|---|---|---|---|
| Catalog No | USB-518598 | ||
| Supplier’s Catalog No | 518598 | ||
| Supplier | US Biologicals | ||
| Source antigen | Lent lily | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 59 | ||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Affinity purified | ||
| Purity | Affinity purified | ||
| Form | Supplied as a lyophilized powder. | ||
| Reactivity life | 2 years | ||
| Note | For reserch purpose only | ||
| Purity | Affinity purified | ||
| Description | Narcissus pseudonarcissus lectin or agglutinin (NPL/NPA) is affinity purified composed of four subunits. It agglutinates rabbit erythrocytes but is non-reactive with human erythrocytes. NPA has an isoelectric point between pH 4.2 and pH 4.6 with a specific binding activity toward alpha-D-mannose, although studies have suggested that the purified lectin may have greater affinity for internal mannosyl residues than for nonreducing terminal units. A buffer washed column elutes the sugar alpha-methylmannoside. Human studies have found NPA to be nearly non-mitogenic, but it has been reported through an in vitro study that mitogenicity of NPA is age-related, with lymphoproliferative response of peripheral blood mononuclear cells (PBMC) in umbilical cord blood has a sevenfold increase than adult blood. NPA has also been found to be mitogenic for PBMC from lambs and not adult sheep. Source: Lent lily Blood Specificity: Rabbit Agglutinin Sugar Specificity: alpha-Mannose Inhibitory Carbohydrate: alpha-Methylmannoside Divalent Ions: None Required Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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