Dolichos biflorus (DBA)
Catalog No : USB-518318
414.95€
0.00€
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| Product name | Dolichos biflorus (DBA) | ||
|---|---|---|---|
| Catalog No | USB-518318 | ||
| Supplier’s Catalog No | 518318 | ||
| Supplier | US Biologicals | ||
| Source antigen | Horse gram | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 111 | ||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Affinity purified | ||
| Purity | Affinity purified | ||
| Form | Supplied as a lyophilized powder. | ||
| Reactivity life | 2 years | ||
| Note | For reserch purpose only | ||
| Purity | Affinity purified | ||
| Description | Dolichos biflorus agglutinin (DBA) is isolated from horse gram and purified by affinity chromatography. DBA is a glycoprotein with a molecular weight of about 111,000 and consists of 4 subunits of equal size. This lectin has an isoelectric point between pH 4.6 and pH 5.5. It has a carbohydrate specificity toward alpha-N-acetylgalactosamine and elutes with the sugar GalNAc. DBA has been used as a general marker of developing renal collecting ducts, which can then be used to establish secretion status in blood group A individuals by hemagglutination inhibition and for detecting blood typing. DBA is over thousand times more active in agglutinating blood group A1 cells versus A2 cells, and is used to distinguish these blood types in routine serology applications, and has potential applications in examining tumors which affect the levels of blood group substances. Reconstitute with sterile buffer. Calcium, manganese, magnesium, and zinc ions are required for binding. Source: Horse gram Blood Specificity: A1 >> A2 Sugar Specificity: alpha-N-Acetylgalactosamine Inhibitory Carbohydrate: N-Acetylgalactosamine Divalent Ions: Ca++, Mn++, Mg++, Zn++ Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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