GALE, Recombinant, Human, aa1-348, His-Tag (DP-glucose 4-epimeraes)

Catalog No : USB-349929
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Product name GALE, Recombinant, Human, aa1-348, His-Tag (DP-glucose 4-epimeraes)
Catalog No USB-349929
Supplier’s Catalog No 349929
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 40.4
Storage -20°C
Other names
Grade Highly Purified
Purity ~95% (SDS-PAGE)
Form Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 5mM DTT, 0.1M sodium chloride, 1mM EDTA, 10% glycerol.
Reactivity life 6 months
Note For reserch purpose only
Purity ~95% (SDS-PAGE)
Description GALE, also known as uDP-glucose 4-epimeraes, is a protein that functions as the third enzyme in the Leloir pathway of galactose metabolism. It is a homodimeric epimerase found in bacterial, plant, and mammalian cells. This enzyme promotes the reverse chemical reaction, the conversion of uDP-glucose to uDP-galactose. uDP-galactose is used to build galactose-containing proteins and fats, which play critical roles in chemical signaling, building cellular structures, transporting molecules, and producing energy. Source: Recombinant protein corresponding to aa1-348 of human GALE, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~40.4kD AA Sequence: MGSSHHHHHH SSGLVPRGSH MAEKVLVTGG AGYIGSHTVL ELLEAGYLPV VIDNFHNAFR GGGSLPESLR RVQELTGRSV EFEEMDILDQ GALQRLFKKY SFMAVIHFAG LKAVGESVQK PLDYYRVNLT GTIQLLEIMK AHGVKNLVFS SSATVYGNPQ YLPLDEAHPT GGCTNPYGKS KFFIEEMIRD LCQADKTWNA VLLRYFNPTG AHASGCIGED PQGIPNNLMP YVSQVAIGRR EALNVFGNDY DTEDGTGVRD YIHVVDLAKG HIAALRKLKE QCGCRIYNLG TGTGYSVLQM VQAMEKASGK KIPYKVVARR EGDVAACYAN PSLAQEELGW TAALGLDRMC EDLWRWQKQN PSGFGTQA Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.