Galectin 8 (GAL8) Recombinant, Human

Catalog No : USB-154721
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Product name Galectin 8 (GAL8) Recombinant, Human
Catalog No USB-154721
Supplier’s Catalog No 154721
Supplier US Biologicals
Source antigen Recombinant Human from E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 38
Storage 4°C/-70°C
Other names
Grade Highly Purified
Purity ≥95%. Endotoxin: ≤1EU/ug (LAL)
Form Supplied as lyophilized form from 20mM Tris, 500mM sodium chloride, pH 8.0, 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose. Reconstitute in sterile PBS, pH 7.3.
Reactivity life 6 months
Note For reserch purpose only
Purity ≥95%. Endotoxin: ≤1EU/ug (LAL)
Description Source: Recombinant protein corressponding to aa1-317 of human Galectin 8 expressed in E. coli with N-terminal His-tag (O00214) Predicted Molecular Weight: 38.0kD Subcellular Location: Cytoplasm Predicted isoelectric point: 8.4 Amino Acid Sequence: MGHHHHHHSGSEFELRRQ-MMLSLNNLQN IIYNPVIPFV GTIPDQLDPG TLIVIRGHVP SDADRFQVDL QNGSSMKPRA DVAFHFNPRF KRAGCIVCNT LINEKWGREE ITYDTPFKRE KSFEIVIMVL KDKFQVAVNG KHTLLYGHRI GPEKIDTLGI YGKVNIHSIG FSFSSDLQST QASSLELTEI SRENVPKSGT PQLRLPFAAR LNTPMGPGRT VVVKGEVNAN AKSFNVDLLA GKSKDIALHL NPRLNIKAFV RNSFLQESWG EEERNITSFP FSPGMYFEMI IYCDVREFKV AVNGVHSLEY KHRFKELSSI DTLEINGDIH LLEVRSW Included Protein Molecular Weight Marker: 168631: Protein Molecular Weight Marker, Unstained Ready to use, No heating, diluting or reducing agents needed. MW: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD, 70kD Double intensity: 10kD, 18kD, 26kD Supplied in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Add 3-5ul/well for mini gels; 7ul/well for large gels. Store at -20°C Stable for 6 months after receipt. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt at -70°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.