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Categories: Peptides & Proteins / Proteins

Product name			Vicia villosa Lectin (VVA, VVL)
Catalog No			USB-V2121-94
Supplier’s Catalog No			V2121-94
Supplier			US Biologicals
Source antigen			Vicia villosa (hairy vetch) seeds
Reactivity
Cross reactivity
Applications
Molecular weight

Storage			-20°C
Other names
Grade			Molecular Biology Grade
Purity			Homogeneous by SDS-PAGE
Form			Supplied as a lyophilized powder.
Reactivity life			12 months
Note			For reserch purpose only
Purity			Homogeneous by SDS-PAGE
Description			This lectin is a family of tetrameric glycoproteins consisting of combinations of A and B subunits similar in structure to PHA and GSL I. The dominant isolectins in our preparations appear to be B subunit-rich. VVL recognizes preferentially alpha- or beta-linked terminal N-acetylgalactosamine, especially a single alpha N-acetylgalactosamine residue linked to serine or threonine in a polypeptide (the “Tn antigen”). Evidence suggests that this lectin also may require specific amino acid sequences at the receptor site of glycosylation. The disaccharide galactosyl (a-1,3) N-acetylgalactosamine is also a potent inhibitor of this lectin. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Inhibiting/Eluting Sugar: 200mM N-acetylgalactosamine Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.