Soybean Agglutinin (SBA, Glycine max)
Catalog No : USB-S5365-30
482.77€
0.00€
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| Product name | Soybean Agglutinin (SBA, Glycine max) | ||
|---|---|---|---|
| Catalog No | USB-S5365-30 | ||
| Supplier’s Catalog No | S5365-30 | ||
| Supplier | US Biologicals | ||
| Source antigen | Glycine max (soybean) seeds | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Molecular Biology Grade | ||
| Purity | |||
| Form | Supplied as a lyophilized powder. Many buffers can be employed for reconstituting and diluting this lectin, we recommend 10mM HEPES buffered saline, pH 8.5, 0.1mM Ca++. For preserving solutions stored at 4°C, 0.08% sodium azide can be used. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | |||
| Description | Composed of four subunits of approximately equal size, soybean agglutinin is a family of closely related isolectins. This glycoprotein has a molecular weight of about 120,000 and an isoelectric point near pH 6.0. SBA preferentially binds to oligosaccharide structures with terminal a- or b-linked N-acetylgalactosamine, and to a lesser extent, galactose residues. Binding can be blocked by substitutions on penultimate sugars, such as fucose attached to the penultimate galactose in blood group B substance. SBA has been used in glycoprotein fractionation, histochemical applications and cell sorter analysis. An important application for SBA is the separation of pluripotential stem cells from human bone marrow. Cells fractionated by SBA do not produce graft vs host disease and can be used in bone marrow transplantation across histocompatibility barriers. It should be noted that some forms of SBA seem to be excellent in separating human cells while others are better for cells of other species. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Inhibiting/Eluting Sugar: 200mM N-acetylgalactosamine Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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