Phaseolus vulgaris L (PHA-L, Red Kidney Bean) (Agarose)
Catalog No : USB-P3370-40A
514.96€
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| Product name | Phaseolus vulgaris L (PHA-L, Red Kidney Bean) (Agarose) | ||
|---|---|---|---|
| Catalog No | USB-P3370-40A | ||
| Supplier’s Catalog No | P3370-40A | ||
| Supplier | US Biologicals | ||
| Source antigen | Phaseolus vulgaris (red kidney bean) seeds | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | 4°C Do Not Freeze | ||
|---|---|---|---|
| Other names | |||
| Grade | Molecular Biology Grade | ||
| Purity | |||
| Form | Supplied as a liquid in 10mM HEPES, pH 7.5, 0.15M sodium chloride, 0.1mM Ca++, 0.08% sodium azide, 0.01mM Mn++. Labeled with Agarose. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | |||
| Description | Phaseolus vulgaris agglutinin is the name ascribed to a family of lectins, each of which consists of four subunits. There are two different types of subunits. One appears to be involved primarily in red cell agglutination and has been designated the “E” subunit (for erythroagglutinin). The other type is involved in lymphocyte agglutination and mitogenic activity and has been termed the “L” subunit (for leucoagglutinin). These subunits combine to produce five isolectins. One of these isolectins has four “E” subunits and is designated PHA-E. PHA-E possesses strong hemagglutinating activity but is a poor mitogen. PHA-L, with four “L” type subunits, does not agglutinate red cells but is a potent mitogen. The other three isolectins, designated E3L1, E2L2, and E1L3, have erythroagglutinating and mitogenic activities proportional to the number of respective “E” or “L” subunits. We have termed the mixture of the five isolectins PHA (E+L). All of these isolectins are glycoproteins with molecular weights of approximately 125,000 and have isoelectric points between pH 5.2 for PHA-L and pH 6.0 for PHA-E. PHA-L, although a potent mitogen, has a rather narrow range of concentrations for mitogenic stimulation and appears to require calcium and manganese ions for activity. The presence of some “E” subunits in PHA appears to broaden the concentration range of maximum mitogenic stimulation. As a consequence, PHA (E+L) may be preferred for general mitogenic assays. PHA-L has been found to be an excellent, specific marker for use in anterograde neuronal tracing. Agarose bound Phaseolus vulgaris Leucoagglutinin (PHA-L) is prepared from affinity-purified lectin. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x10e7 are used as the solid-phase matrix to which the lectin is covalently bound. The attachment of the lectin to the solid phase is carefully controlled in order to preserve the activity of the lectin as well as to minimize conformational changes of the bound lectin which might result in nonspecific ionic or hydrophobic interactions. The technique developed to couple lectins to agarose provides a very hydrophilic spacer arm between the protein and the matrix. This ensures maximum expression of the carbohydrate binding activity of the lectin. The linkage is very stable over a range of pH values and, unlike cyanogen bromide linkages, proteins are not leached off the gel by Tris or other routinely used buffers. In addition, residual charges generated during cyanogen bromide conjugation which can produce nonspecific binding are not present on the gel following our coupling procedure. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Binding Capacity: ≥4.0mg thyroglobulin/ml of gel Elution: 100mM acetic acid 3mg lectin/ml gel Activity: 2x10e7 Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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