Narcissus pseudonarcissus Lectin (NPA, NPL)
Catalog No : USB-N0204-10
482.77€
0.00€
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| Product name | Narcissus pseudonarcissus Lectin (NPA, NPL) | ||
|---|---|---|---|
| Catalog No | USB-N0204-10 | ||
| Supplier’s Catalog No | N0204-10 | ||
| Supplier | US Biologicals | ||
| Source antigen | Daffodil bulbs | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Molecular Biology Grade | ||
| Purity | Homogeneous by SDS-PAGE | ||
| Form | Supplied as a lyophilized powder. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | Homogeneous by SDS-PAGE | ||
| Description | Daffodil lectin exists as a dimeric protein composed of two subunits of 13,000D devoid of attached carbohydrate. Below pH 5.0 and above pH 9.0 NPL dissociates into monomers. This lectin has a specificity toward alpha linked mannose, preferring polymannose structures containing (a-1,6) linkages. Binding to mannose polymers can occur via internal mannose residues and is not dependent on structural integrity of a non-reducing end sugar. NPL also binds some galactomannans, and differs in other binding characteristics from a related lectin, Galanthus nivalis lectin. Unlike Con A, LCA and PSA, NPL does not bind glucose. Staining of the cytoplasmic membrane of cells with NPL after short-term acid treatment can indicate those cells undergoing apoptosis. Detection of apoptotic cells using NPL binding is a highly sensitive and reliable tool for studying cell death. Applications: User-determined Inhibiting Sugar: 100mM mannose Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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