Maclura pomifera Lectin (MPL) (Biotin)
Catalog No : USB-M1104-10B
482.77€
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| Product name | Maclura pomifera Lectin (MPL) (Biotin) | ||
|---|---|---|---|
| Catalog No | USB-M1104-10B | ||
| Supplier’s Catalog No | M1104-10B | ||
| Supplier | US Biologicals | ||
| Source antigen | Maclura pomifera (Osage orange tree) seeds | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Molecular Biology Grade | ||
| Purity | |||
| Form | Supplied as a lyophilized powder in 10mM HEPES, pH 7.5, 0.15M sodium chloride, 0.08% sodium azide. Reconstitute with 1ml dH2O. Labeled with Biotin. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | |||
| Description | This lectin has a molecular weight of 40,000-46,000 and is composed of four subunits. There appear to be two different chains in combinations giving rise to five isolectins. The isoelectric point of this lectin is pH 4.7, and it is probably devoid of carbohydrate or metal ions. The carbohydrate specificity of either type of subunit is likely to be the same, preferring alpha linked N-acetylgalactosamine structures. This biotinylated lectin conjugate is prepared from affinity-purified lectin and is optimally labeled with biotin. Essentially free of inactive lectin conjugate and containing no free biotin, this biotinylated lectin provides an ideal intermediate for examining glycoconjugates using the Biotin-Avidin System. First the biotin-labeled lectin is added, followed by an ABC Reagent, Avidin D conjugate, or streptavidin derivative. Another possible application is in the isolation of lymphokines and other products of mitogenic stimulation. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Inhibiting Sugar: 500mM galactose Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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