Maackia amurensis Lectin II (MAL II)

Catalog No : USB-M1061-15
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Product name Maackia amurensis Lectin II (MAL II)
Catalog No USB-M1061-15
Supplier’s Catalog No M1061-15
Supplier US Biologicals
Source antigen Maackia amurensis seeds
Reactivity
Cross reactivity
Applications
Molecular weight
Storage 4°C
Other names
Grade Molecular Biology Grade
Purity Homogeneous by SDS-PAGE
Form Supplied as a liquid in 10mM HEPES, pH 7.5, 0.15M sodium chloride, 0.08% sodium azide, 0.1mM Ca2+.
Reactivity life 12 months
Note For reserch purpose only
Purity Homogeneous by SDS-PAGE
Description Maackia amurensis lectin II (MAH) is the hemagglutinin isolectin from Maakia seeds. It is a glycoprotein consisting of four subunits. The molecular weight of the lectin is about 140,000 resolvable by SDS gel electrophoresis into two bands of 34,000 and 36,000D. The isoelectric point of this lectin is about pH 4.7 but demonstrates some charge heterogeneity. Although the specificity of this lectin is not well defined, MAL II appears to bind only particular carbohydrate structures that contain sialic acid. Unlike SNA which seems to prefer structures with (a-2,6) linked sialic acid, MAL II appears to bind sialic acid in an (a-2,3) linkage. While fetuin is a poor inhibitor, glycophorin is a very potent inhibitor of MAL II binding. Tissue staining patterns are also very different among MAL I, SNA and MAL II. Applications: User-determined Inhibitor: Human glycophorin Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.