Maackia amurensis Lectin I (MAL I) (FITC)
Catalog No : USB-M1061-10F
477.02€
0.00€
Shipping cost plus VAT not included , delivery in 7-14 business days
| Product name | Maackia amurensis Lectin I (MAL I) (FITC) | ||
|---|---|---|---|
| Catalog No | USB-M1061-10F | ||
| Supplier’s Catalog No | M1061-10F | ||
| Supplier | US Biologicals | ||
| Source antigen | Maackia amurensis seeds | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Molecular Biology Grade | ||
| Purity | |||
| Form | Supplied as a liquid in 10mM HEPES, 0.15M sodium chloride, pH 7.5, 0.08% sodium azide. 0.1mM Ca++. Labeled with Fluorescein isothiocyanate (FITC). | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | |||
| Description | This lectin (MAL) is the leucoagglutinin or mitogenic isolectin form from Maackia seeds. This multimeric lectin dissociates into 38,000 and 40,000 molecular weight bands in SDS gels in the presence of reducing agents and is a single 75,000D band without reducing agents. It has an isoelectric point about pH 4.6 and has been reported to have a molecular weight of about 130,000 by gel filtration. This lectin binds glycoconjugates having galactosyl (b-1,4) N-acetylglucosamine structures. Maackia amurensis lectin I seems to tolerate substitution of N-acetyllactosamine with sialic acid at the 3 position of galactose. However, MAL I does not appear to bind this structure when substitution with sialic acid is on the 6 position of galactose. Fluorescein labeled Maackia Amurensis Lectin is produced by using the highest quality fluorescein isothiocyanate, our affinity-purified lectin, and special conjugation procedures. Fluorescein labeled Maackia Amurensis Lectin has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This lectin is supplied essentially free of unconjugated fluorochromes and inactive lectin. Applications: Suitable for use in Immunofluorescence. Other applications not tested. Recommended Dilutions: Immunofluorescence: 5-20ug/ml. Optimal dilutions to be determined by the researcher. Inhibiting Sugar: 200mM lactose Sugar Specificity: Galatose, lactose Excitation: 495-500nm Emission: 514-521nm F/P (molar): 9.1 Active Conjugate: As Reported Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
Product Details
© 2020 Imugex All Rights Reserved