Lens culinaris Agglutinin (LCA) (Agarose)
Catalog No : USB-L1665-15A
463.23€
0.00€
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| Product name | Lens culinaris Agglutinin (LCA) (Agarose) | ||
|---|---|---|---|
| Catalog No | USB-L1665-15A | ||
| Supplier’s Catalog No | L1665-15A | ||
| Supplier | US Biologicals | ||
| Source antigen | |||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | 4°C Do Not Freeze | ||
|---|---|---|---|
| Other names | |||
| Grade | Molecular Biology Grade | ||
| Purity | |||
| Form | Supplied as a liquid in 10mM HEPES, pH 7.5, 0.15M sodium chloride, 0.1mM Ca++, 0.08% sodium azide, 10mM Mannose, 0.01mM Mn++. Labeled with Agarose. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | |||
| Description | Lens culinaris agglutinin has a molecular weight of ~49kD and is composed of four subunits-two of ~17,kD and two of 8kD. The isoelectric point of LCA is ~pH 8.5. LCA recognizes sequences containing a-linked mannose residues. Like other mannose specific lectins, divalent cations such as calcium and manganese are required for sugar binding activity. By recognizing additional sugars as part of the receptor structure, LCA has a narrower specificity than Con A. For example, an a-linked fucose residue attached to the N-acetylchitobiose portion of the core oligosaccharide markedly enhances affinity. By exploiting this narrower specificity, glycoproteins and glycopeptides can be subfractionated with LCA after initial isolation with Con A. LCA has been employed to separate lymphocyte populations, as a potent T-cell mitogen, and as one of the most effective agents in preventing skin allograft rejection in model systems. LCA is also used to purify numerous glycoproteins (including immunoglobulins, histocompatibility antigens, a2-macroglobulin, etc.) as well as to fractionate glycopeptides from a variety of glycoproteins. Agarose bound Lens Culinaris Agglutinin is prepared from affinity-purified lectin. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x10e7 are used as the solid-phase matrix to which the lectin is covalently bound. The attachment of the lectin to the solid phase is carefully controlled in order to preserve the activity of the lectin as well as to minimize conformational changes of the bound lectin which might result in nonspecific ionic or hydrophobic interactions. Binding Capacity: ≥3.0mg mannosyl glycoprotein/ml gel Inhibiting/Eluting Sugar: 200mM a-methyl mannoside/200mM a-methyl glucoside mixture 3mg lectin/ml gel Activity: 2x10e7 Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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