Hippeastrum hybrid Lectin (HHL, Amaryllis Lectin, AL) (Biotin)
Catalog No : USB-H4066-75B
463.23€
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| Product name | Hippeastrum hybrid Lectin (HHL, Amaryllis Lectin, AL) (Biotin) | ||
|---|---|---|---|
| Catalog No | USB-H4066-75B | ||
| Supplier’s Catalog No | H4066-75B | ||
| Supplier | US Biologicals | ||
| Source antigen | Amaryllis bulbs | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Molecular Biology Grade | ||
| Purity | |||
| Form | Supplied as a lyophilized powder in 10mM HEPES, pH 7.5, 0.15M sodium chloride, 0.08% sodium azide. Reconstitute with 1ml dH2O. Labeled with Biotin. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | |||
| Description | This 50kD tetrameric lectin is composed of four subunits of identical size and has an acidic isoelectric point. This lectin does not contain attached carbohydrate and is immunologically related to GNL and NPL. Amaryllis lectin binds only alpha mannose residues, not alpha glucosyl structures in contrast to Con A, PSA and LCA. Like NPL there appears to be an extended binding site for polymannose structures, not requiring mannose to be at the non-reducing terminus. HHL binds both (a -1,3) and (a-1,6) linked mannose structures, as well as some yeast galactomannans. This biotinylated lectin conjugate is prepared from affinity-purified lectin and is optimally labeled with biotin. Essentially free of inactive lectin conjugate and containing no free biotin, this biotinylated lectin provides an ideal intermediate for examining glycoconjugates using the Biotin-Avidin System. First the biotin-labeled lectin is added, followed by an ABC Reagent, Avidin D conjugate, or streptavidin derivative. Another possible application is in the isolation of lymphokines and other products of mitogenic stimulation. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Inhibiting Sugar: 100mM α-methylmannoside Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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