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Categories: Peptides & Proteins / Proteins

Product name			Hippeastrum hybrid Lectin (HHL, Amaryllis Lectin, AL)
Catalog No			USB-H4066-75
Supplier’s Catalog No			H4066-75
Supplier			US Biologicals
Source antigen
Reactivity
Cross reactivity
Applications
Molecular weight

Storage			-20°C
Other names
Grade			Molecular Biology Grade
Purity			Purified by SDS-PAGE
Form			Supplied as a lyophilized powder, with ~15% salts. We recommend using 10mM HEPES buffered saline, pH 8.5, 0.1mM Ca++ for reconstitution and diluting. For preserving solutions at 4ºC, 0.08% sodium azide should be used.
Reactivity life			12 months
Note			For reserch purpose only
Purity			Purified by SDS-PAGE
Description			This 50kD tetrameric lectin is composed of four subunits of identical size and has an acidic isoelectric point. This lectin does not contain attached carbohydrate and is immunologically related to GNL and NPL. Amaryllis lectin binds only alpha mannose residues, not alpha glucosyl structures in contrast to Con A, PSA and LCA. Like NPL there appears to be an extended binding site for polymannose structures, not requiring mannose to be at the non-reducing terminus. HHL binds both (a -1,3) and (a-1,6) linked mannose structures, as well as some yeast galactomannans. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.