Griffonia simplicifolia Lectin I Isolectin B4 (Bandeiraea simplicifolia) (GSL I–isolectin B4, GS I-B4) (FITC)

Catalog No : USB-G8967-93F
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Product name Griffonia simplicifolia Lectin I Isolectin B4 (Bandeiraea simplicifolia) (GSL I–isolectin B4, GS I-B4) (FITC)
Catalog No USB-G8967-93F
Supplier’s Catalog No G8967-93F
Supplier US Biologicals
Source antigen
Reactivity
Cross reactivity
Applications
Molecular weight
Storage -20°C
Other names
Grade Molecular Biology Grade
Purity
Form Supplied as a liquid in 10mM HEPES, 0.15M sodium chloride, pH 7.5, 0.08% sodium azide, 0.1mM Ca++. Labeled with Fluorescein.
Reactivity life 12 months
Note For reserch purpose only
Purity
Description GSL I-B4 isolectin contains only the B subunits and has been reported to be useful as a marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat. Fluorescein labeled GSL I – isolectin B4 is produced by using the highest quality fluorescein isothiocyanate, our affinity-purified lectin, and special conjugation procedures. Fluorescein labeled GSL I – isolectin B4 has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This lectin is supplied essentially free of unconjugated fluorochromes and inactive lectin. Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions perserved with sodium azide. Applications: Suitable for use in FLISA. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Inhibiting Sugar: 500mM galactose or 100mM raffinose F/P (molar): 3.1 Excitation: 495-500nm Emission: 515-521nm Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 6 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.