Griffonia simplicifolia Lectin I Isolectin B4 (Bandeiraea simplicifolia) (GSL I–isolectin B4, GS I-B4) (Biotin)
Catalog No : USB-G8967-93B
467.83€
0.00€
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| Product name | Griffonia simplicifolia Lectin I Isolectin B4 (Bandeiraea simplicifolia) (GSL I–isolectin B4, GS I-B4) (Biotin) | ||
|---|---|---|---|
| Catalog No | USB-G8967-93B | ||
| Supplier’s Catalog No | G8967-93B | ||
| Supplier | US Biologicals | ||
| Source antigen | |||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Molecular Biology Grade | ||
| Purity | |||
| Form | Supplied as a liquid in 10mM HEPES, pH 7.5, 0.15M sodium chloride, 0.08% sodium azide, 0.1mM Ca++. Labeled with Biotin. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | |||
| Description | GSL I-B4 isolectin contains only the B subunits and has been reported to be useful as a marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat. This biotinylated lectin conjugate is prepared from affinity-purified lectin and is optimally labeled with biotin. Essentially free of inactive lectin conjugate and containing no free biotin, this biotinylated lectin provides an ideal intermediate for examining glycoconjugates using the Biotin-Avidin System. First the biotin-labeled lectin is added, followed by an ABC Reagent, Avidin D conjugate, or streptavidin derivative. Another possible application is in the isolation of lymphokines and other products of mitogenic stimulation. Applications: Suitable for use in ELISA, Western Blot and Histochemistry. Other applications not tested. Recommended Dilution: ELISA: 5-20 ug/ml Histochemistry (paraffin or frozen sections): 5-20 ug/ml Western Blot: 5-20 ug/ml Optimal dilutions to be determined by the researcher. Inhibiting Sugar: 500mM galactose or 100mM raffinose Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in above buffer. | ||
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