Griffonia simplicifolia GS I (Bandeiraea simplicifolia)

Catalog No : USB-G8967-12
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Product name Griffonia simplicifolia GS I (Bandeiraea simplicifolia)
Catalog No USB-G8967-12
Supplier’s Catalog No G8967-12
Supplier US Biologicals
Source antigen Griffonia (Bandeiraea) simplicifolia seeds
Reactivity
Cross reactivity
Applications
Molecular weight
Storage -20°C
Other names
Grade Molecular Biology Grade
Purity Homogeneous by SDS-PAGE
Form Supplied as a lyophilized powder.
Reactivity life 12 months
Note For reserch purpose only
Purity Homogeneous by SDS-PAGE
Description GSL I is a family of glycoproteins with molecular weights of approximately 115,000. There are two types of subunits, termed “A” and “B”, with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The “A”-rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for a-N-acetylgalactosamine residues, while the “B”-rich lectin preferentially agglutinates blood group B cells and is specific for a-galactose residues. Our GSL I is a mixture of the five isolectins. Binding appears to require divalent cations such as calcium and magnesium. GSL I has been reported to bind several glycoproteins including laminin. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Inhibiting/Eluting Sugar: 200mM galactose/200mM N-acetylgalactosamine mixture Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.