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Categories: Peptides & Proteins / Proteins

Product name			Bauhinia purpurea Lectin (BPL)
Catalog No			USB-B0160
Supplier’s Catalog No			B0160
Supplier			US Biologicals
Source antigen			Bauhinia purpurea alba seeds
Reactivity
Cross reactivity
Applications
Molecular weight

Storage			-20°C
Other names
Grade			Molecular Biology Grade
Purity			Homogeneous by SDS-PAGE
Form			Supplied as a lyophilized powder in ~18% salts. We recommend using 10mM HEPES buffered saline, pH 8.5, 0.1mM Ca++ for reconstitution and diluting. For preserving solutions at 4ºC, 0.08% sodium azide should be used.
Reactivity life			12 months
Note			For reserch purpose only
Purity			Homogeneous by SDS-PAGE
Description			This tetrameric lectin consists of 44,000D subunits and does not require divalent cations for binding. Binding appears to be highest for glycoconjugates containing galactosyl (b-1,3) N-acetylgalactosamine structures but oligosaccharides with a terminal alpha linked N-acetylgalactosamine can also bind. Although binding specificity is similar to that of peanut agglutinin, tissue staining patterns of these two lectins are distinct. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Inhibiting/Eluting Sugar: 100mM lactose Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.