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Categories: Peptides & Proteins / Proteins

Product name			Griffonia (Bandeiraea) simplicifolia GS I A4 (GS-I A4) (FITC)
Catalog No			USB-G8967-44
Supplier’s Catalog No			G8967-44
Supplier			US Biologicals
Source antigen
Reactivity
Cross reactivity
Applications
Molecular weight

Storage			-20°C
Other names
Grade			Purified
Purity
Form			Supplied as a liquid in PBS, 0.15M sodium chloride, 0.5mM calcium chloride, pH 7.3, 0.05% sodium azide. Labeled with FITC.
Reactivity life			12 months
Note			For reserch purpose only
Purity
Description			Pure Griffonia simplicifolia GS I A4 lectin, FITC conjugated. Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose. FITC/Protein Ratio (OD495/OD280): 1.73 Carbohydrate Specificity: a-GalNAc Inhibitory Carbohydrate: N-Acetylgalactosamine > Gal a(1,3) Gal=Methyl-a-Galactoside > Galactose > Methyl-b-Galactoside. Activity: 20-30ug/ml is required to agglutinate fresh type A1 blood cells. Lectin activity against all blood types increases after neuraminidase treatment of the cells. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.