PVL LukF, N-terminus, Recombinant, His-tagged
Catalog No : USB-299604
498.86€
0.00€
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| Product name | PVL LukF, N-terminus, Recombinant, His-tagged | ||
|---|---|---|---|
| Catalog No | USB-299604 | ||
| Supplier’s Catalog No | 299604 | ||
| Supplier | US Biologicals | ||
| Source antigen | Staphylococcus aureus | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 37 | ||
| Storage | -70°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Purified | ||
| Purity | Purified | ||
| Form | Supplied as a liquid in PBS, 10% glycerol. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | Purified | ||
| Description | Purified, His-tagged Staphylococcus aureus Panton-Valentine Leukocidin (PVL) F subunit (LukF-PV). This protein may be used in functional PVL toxicity assays in combination with LukS-PV, or as a control protein in ELISA assays or Western blotting when detecting LukF in PVL (+) strains of S. aureus. Molecular Weight: Calculated: 35kD, including His tag Observed: ~37kD (SDS-PAGE) Applications: Suitable for use in ELISA, Western Blot and Cytotoxicity Assay. Other applications not tested. Recommended Dilutions: Western Blot: Internal QC showed detection of 100ng of LukF-PV protein when detected with monoclonal anti-LukF antibody (Cat. #299602). Cytotoxicity Assay: 1-100nM using human neutrophils Optimal dilutions to be determined by the researcher. Cytotoxicity Assay: Cytotoxicity can be detected in human neutrophils when used in combination with LukS-PV in a concentration range of 1-100nM. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | ||
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