HIV-1, CM, gp120, Recombinant (Human Immunodeficiency Virus-1)

Catalog No : USB-209002
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Product name HIV-1, CM, gp120, Recombinant (Human Immunodeficiency Virus-1)
Catalog No USB-209002
Supplier’s Catalog No 209002
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 100
Storage -20°C
Other names
Grade Highly Purified
Purity ~95% (10% PAGE, coomassie staining, RP-HPLC)
Form Supplied as a lyophilized powder in 25mM Tris, 250mM glycine, 0.1% SDS. Reconstitute with 100ul sterile ddH2O for a ~1mg/ml stock.
Reactivity life 12 months
Note For reserch purpose only
Purity ~95% (10% PAGE, coomassie staining, RP-HPLC)
Description Human T-lymphotropic virus (HTLV) is a human, single-stranded RNA retrovirus that causes T-cell leukemia and T-cell lymphoma. The virus activates a subset of T-helper cells called Th1 cells. The result is a proliferation of Th1 cells and overproduction of Th1 related cytokines (mainly IFN-gamma and TNF-alpha). Feedback mechanisms of these cytokines cause a suppression of the Th2 lymphocytes and a reduction of Th2 cytokine production (mainly IL- 4, IL-5, IL-10 and IL-13). The end result is a reduction in the ability of the infected host to mount an adequate immune response to invading organisms that require a predominantly Th2 dependant response (these include parasitic infections and production of mucosal and humoral antibodies). Source: Recombinant protein corresponding to HIV-1, strain: BL21 DE3, expressed in E. coli. Molecular Weight: ~100kD Applications: Suitable for use as an antigen in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.