Imugex

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Categories: Peptides & Proteins / Proteins

Product name			Herpes Simplex Virus I (HSV I, Herpes Simplex Virus 1, HSV1)
Catalog No			USB-H2033-03H
Supplier’s Catalog No			H2033-03H
Supplier			US Biologicals
Source antigen			Crude lysate
Reactivity
Cross reactivity
Applications
Molecular weight

Storage			-70°C
Other names
Grade			Purified
Purity			Purified
Form			Supplied as a liquid in PBS, 0.1% Triton X-100.
Reactivity life			12 months
Note			For reserch purpose only
Purity			Purified
Description			Preparation: HSV-1 is propagated in mammalian cells using a protocol optimized for maximum particle yield. At the appropriate time, infected cells are washed in saline then disrupted to release virus particles by freeze thaw and sonication. Purification and presentation: Virus particles released from cells are partially purified by low speed centrifugation to remove cell debris. HSV-1 particles are then purified by sucrose density gradient. This process also includes a DNase step to remove cellular DNA and helps to achieve a high level of purity. Source: HSV, strain 1, from a crude lysate. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Inactivation: 0.1% Triton X-100. Tested by passage on permissive cells for 5 days yielding no CPE. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: No test guarantees a product to be non-infectious. All materials should be handled as if potentially infectious. Generally accepted laboratory practices appropriate for infectious materials should be employed when handling this product.