violetFluor™ 450 Human CD27 (O323)
Catalog No : ABM-Y600560
317.80€
0.00€
Shipping cost plus VAT not included , delivery in 7-14 business days
| Description | The O323 antibody reacts with human CD27 (TNFRSF7), a cell surface homodimer of 55 kDa subunits, which provides co-stimulatory signaling in support of the T cell (TCR) and B cell (BCR) receptors. By comparison with CD28, whose TCR co-stimulatory signal can trigger cell proliferation, CD27 signaling appears to promote cell survival and differentiation to effector / memory stages. Also in contrast with CD28, the CD27 receptor may be shed following interaction with its ligand CD70, which is typically expressed on activated dendritic cells, T cells and B cells. With respect to B cells, CD27 is considered to be a phenotypic marker for memory B cells. CD27 has been included within a group of phenotypic markers for identifying human B regulatory cells (Bregs), a cell type proposed to regulate CD4+ T cell proliferation and Foxp3 / CTLA-4 expression in Treg cells.The O323 antibody may be used for analysis of CD27 expression on peripheral T cells, and is frequently used in combination with antibodies for IgD and IgM to distinguish nave vs. memory B cell populations. For identification of Breg cells, this antibody has been used in combination with antibodies for CD25, CD1d, IL-10 and TGF-beta (Kessel et al. 2012. Autoimm. Rev. 11(9): 670-677). The antibody is also reported for cross-reactivity with Baboon, Cynomolgus and Rhesus CD27. | ||
|---|---|---|---|
| Other names | |||
| Cellular localization | |||
| Research area | |||
| Note | This product is for research purpose only, not for use in diagnostics or as therapeutics. | ||
| Uniprot ID | |||
| Product name | violetFluor™ 450 Human CD27 (O323) | ||
|---|---|---|---|
| Catalog No | ABM-Y600560 | ||
| Supplier’s Catalog No | Y600560 | ||
| Supplier | Applied Biological Materials | ||
| Host species | Mouse | ||
| Species reactivity | Human, Baboon, Cynomolgus, Rhesus | ||
| Applications | Flow cyto | ||
| Recommended dilutions | Optimal dilution should be determined by the researcher. | ||
| Molecular weight | |||
| Conjugate | Fluor 450 | ||
| Immunogen | N/A | ||
| Specificity | |||
| Storage | This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid repeated freeze and thaw cycles. | ||
|---|---|---|---|
| Clonality | Monoclonal | ||
| Isotype | Mouse IgG1, kappa | ||
| Clone ID | |||
| Concentration | It may differ from batch to batch. Please contact us to confirm it. | ||
| Storage buffer | 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3,0.1% gelatin, pH7.2 | ||
| Formulation | Liquid | ||
| Purification | Affinity Purified | ||
| Handling | This product contains sodium azide (NaN3) as preservative, please take appropriate care when handling this product. | ||
| Reactivity life | One year when stored properly according to instructions. | ||
Product Details
© 2020 Imugex All Rights Reserved