Anti-RAN Antibody
Catalog No : IGX-50028
237.00€
175.00€
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| Description | GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs (PMID:10400640, PMID:8276887, PMID:8896452, PMID:8636225, PMID:8692944, PMID:9351834, PMID:9428644, PMID:9822603, PMID:26272610). Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis (PMID:7819259, PMID:8896452, PMID:8636225, PMID:8692944, PMID:9351834, PMID:9428644, PMID:9822603, PMID:29040603, PMID:11336674, PMID:26272610). Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN, while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensures the directionality of the transport (PMID:8896452, PMID:9351834, PMID:9428644). Interaction with RANBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins (PMID:20485264). RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation (PMID:10408446, PMID:29040603). Required for normal progress through mitosis (PMID:8421051, PMID:12194828, PMID:29040603). The complex with BIRC5/survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PMID:18591255). Acts as a negative regulator of the kinase activity of VRK1 and VRK2 (PMID:18617507). Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases (PMID:10400640). | ||
|---|---|---|---|
| Other names | GTP-binding nuclear protein Ran, Androgen receptor-associated protein 24, GTPase Ran, Ras-like protein TC4, Ras-related nuclear protein, RAN, ARA24 | ||
| Cellular localization | Cytosol, Nucleus, Nucleus Envelope, Cytoplasm, Melanosome | ||
| Research area | Cell Biology | ||
| Note | This product is for research purpose only, not for use in diagnostics or as therapeutics. | ||
| Uniprot ID | P62826 | ||
| Product name | Anti-RAN Antibody | ||
|---|---|---|---|
| Catalog No | IGX-50028 | ||
| Supplier’s Catalog No | IGX-50028 | ||
| Supplier | ImuGeX | ||
| Host species | Rabbit | ||
| Species reactivity | Human | ||
| Applications | ELISA, WB, IHC | ||
| Recommended dilutions | WB: 1:500-1:5000, IHC: 1:20-1:200 | ||
| Molecular weight | Predicted band size: 24 KDa. Observed band size: 24 KDa. | ||
| Conjugate | Unconjugated | ||
| Immunogen | Recombinant Human GTP-binding nuclear protein Ran protein (1-216AA) | ||
| Specificity | RAN antibody detects endogenous level of RAN protein | ||
| Storage | Store at +4°C for short term (1 week), for long term aliquote and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles, with each freeze and thaw cycle an antibody can lose half of its binding activity. | ||
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| Clonality | Polyclonal | ||
| Isotype | IgG | ||
| Clone ID | N/A | ||
| Concentration | Concentration can vary between different batches. | ||
| Storage buffer | Phosphate-buffered saline (pH 7.3) containing 0.02% Sodium Azide (NaN3) and 50% Glycerol. | ||
| Formulation | Liquid | ||
| Purification | This antibody is immunogen affinity purified. | ||
| Handling | This product contains sodium azide (NaN3) as preservative, please take appropriate care when handling this product. | ||
| Reactivity life | 12 months when stored properly according to instructions (provided antibody remains sterile). | ||
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