Anti-PDCD1 Antibody
Catalog No : IGX-47437
262.00€
188.00€
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| Description | Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PMID:21276005). Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PMID:21276005). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation . Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta . The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PMID:28951311). The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function (PMID:28951311). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (PMID:22658127, PMID:25034862, PMID:25399552). | ||
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| Other names | Programmed cell death protein 1, Protein PD-1, hPD-1, CD antigen CD279, PDCD1, PD1 | ||
| Cellular localization | Plasma Membrane, Membrane, Single-Pass Type I Membrane | ||
| Research area | Cell Biology | ||
| Note | This product is for research purpose only, not for use in diagnostics or as therapeutics. | ||
| Uniprot ID | Q15116 | ||
| Product name | Anti-PDCD1 Antibody | ||
|---|---|---|---|
| Catalog No | IGX-47437 | ||
| Supplier’s Catalog No | IGX-47437 | ||
| Supplier | ImuGeX | ||
| Host species | Rabbit | ||
| Species reactivity | Human, Mouse | ||
| Applications | ELISA, WB, IHC, IF | ||
| Recommended dilutions | WB: 1:1000-1:5000, IHC: 1:20-1:200, IF: 1:50-1:200 | ||
| Molecular weight | Predicted band size: 32 KDa. Observed band size: 32 KDa. | ||
| Conjugate | Unconjugated | ||
| Immunogen | Recombinant Human Programmed cell death protein 1 protein (21-170AA) | ||
| Specificity | PDCD1 antibody detects endogenous level of PDCD1 protein | ||
| Storage | Store at +4°C for short term (1 week), for long term aliquote and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles, with each freeze and thaw cycle an antibody can lose half of its binding activity. | ||
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| Clonality | Polyclonal | ||
| Isotype | IgG | ||
| Clone ID | N/A | ||
| Concentration | 1 mg/mL | ||
| Storage buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. | ||
| Formulation | Liquid | ||
| Purification | This antibody is immunogen affinity purified. | ||
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. | ||
| Reactivity life | 12 months when stored properly according to instructions (provided antibody remains sterile). | ||
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