Zinc-alpha-2-Glycoprotein, Recombinant, Human (ZAG2)
Catalog No : USB-299569
419.55€
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| Product name | Zinc-alpha-2-Glycoprotein, Recombinant, Human (ZAG2) | ||
|---|---|---|---|
| Catalog No | USB-299569 | ||
| Supplier’s Catalog No | 299569 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 30.25 | ||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~98% (RP-HPLC, SDS-PAGE) | ||
| Form | Supplied as a lyophilized powder. No preservative added. Reconstitute with 100ug/ml sterile ddH2O. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Description | Zinc-alpha-2-glycoprotein (ZAG) is found in body fluids such as serum, sweat, and seminal and breast cyst fluids. It is identical in amino acid sequence to tumor-derived lipid mobilizing factor (LMF), a protein associated with the dramatic loss of adipose body stores in cancer cachexia, and has been shown to stimulate lipolysis by adipocytes in vivo and in vitro. A role for ZAG has been proposed in the regulation of body weight, and age-dependent changes in genetically influenced obesity, and also it regulates melanin production by normal and malignant melanocytes. It has also recently been classified as a novel adipokine in that it is produced by both white and brown fat adipocytes and may be useful for the study of Diabetes and obesity. Source: Recombinant protein corresponding to human ZAG2, a homodimeric, non-glycosylated polypeptide chain containing a total of 264aa, expressed in E. coli. Molecular Weight: ~30.25kD Biological Activity: The ED50, as determined by the dose-dependant induction of IL-6 in primary human foreskin fibroblasts, was found to be 2ng/ml, corresponding to a specific activity of 5x10e5units/mg. AA Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Ile-Thr-Ile-Pro. Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm using the absorbency value of 1.13 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-17 as a Reference Standard. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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