Anti-ALOX5 Antibody
Catalog No : IGX-43602
262.00€
188.00€
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| Description | Catalyzes the oxygenation of arachidonic acid to 5-hydroperoxyicosatetraenoic acid (5-HPETE) followed by the dehydration of the hydroperoxide into an epoxide, 5,6- oxidoicosatetraenoic acid (LTA4), thereby participates in the first step in leukotriene biosynthesis and in the inflammatory processes (PMID:8631361, PMID:21233389, PMID:22516296, PMID:24282679, PMID:19022417, PMID:23246375, PMID:8615788, PMID:24893149, PMID:31664810). Also catalyzes the oxygenation of arachidonic acid into 8-hydroperoxyicosatetraenoic acid (8-HPETE) and 12-hydroperoxyicosatetraenoic acid (12-HPETE) (PMID:23246375). Although arachidonic acid is the preferred substrate, can also metabolize oxidized fatty acids derived from arachidonic acid such as (15S)-HETE, eicosapentaenoic acid (EPA) such as (18R)- and (18S)-HEPE or docosahexaenoic (DHA) acid which leads to the formation of specialized pro-resolving mediators (SPM) lipoxin and resolvins E and D respectively, therefore plays a role in anti-inflammatory response (PMID:21206090, PMID:31664810, PMID:8615788, PMID:17114001). Also displays lipoxin synthase activity being able to convert (15S)-HETE into a conjugate tetraene (PMID:31664810). Oxidation of docosahexaenoic acid directly inhibits endothelial cell proliferation and sprouting angiogenesis via peroxisome proliferator-activated receptor gamma (PPARgamma) . Does not catalyze the oxygenation of linoleic acid and does not convert (5S)-HETE to lipoxins isomers (PMID:31664810). In addition to inflammatory processes, participates in dendritic cell migration, wound healing through an antioxidant mechanism based on HO1 regulation expression, monocyte adhesion to the endothelium via ITGAM expression on monocytes . Moreover, establishes an adaptive humoral immunity by regulating primary resting B cells and follicular helper T cells and participates in the CD40-induced production of reactive oxygen species (ROS) after CD40 ligation in B cells through interaction with PIK3R1 that bridges ALOX5 with CD40 (PMID:21200133). Also may play a role in glucose homeostasis, regulation of INS secretion and palmitic acid-induced insulin resistance via AMPK . Can regulate bone mineralization and fat cell differentiation increases in induced pluripotent stem cells . | ||
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| Other names | Arachidonate 5-lipoxygenase, 5-LO, 5-lipoxygenase, EC 1.13.11.34, ALOX5, LOG5 | ||
| Cellular localization | Cytosol, Nucleus Matrix, Nucleus Membrane, Peripheral Membrane, Nucleus Envelope, Nucleus Intermembrane Space, Cytoplasm, Perinuclear Region | ||
| Research area | Signal Transduction | ||
| Note | This product is for research purpose only, not for use in diagnostics or as therapeutics. | ||
| Uniprot ID | P09917 | ||
| Product name | Anti-ALOX5 Antibody | ||
|---|---|---|---|
| Catalog No | IGX-43602 | ||
| Supplier’s Catalog No | IGX-43602 | ||
| Supplier | ImuGeX | ||
| Host species | Rabbit | ||
| Species reactivity | Human | ||
| Applications | ELISA, IHC, IF | ||
| Recommended dilutions | IHC: 1:20-1:200, IF: 1:50-1:200 | ||
| Molecular weight | N/A | ||
| Conjugate | Unconjugated | ||
| Immunogen | Recombinant Human Arachidonate 5-lipoxygenase protein (2-149AA) | ||
| Specificity | ALOX5 antibody detects endogenous level of ALOX5 protein | ||
| Storage | Store at +4°C for short term (1 week), for long term aliquote and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles, with each freeze and thaw cycle an antibody can lose half of its binding activity. | ||
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| Clonality | Polyclonal | ||
| Isotype | IgG | ||
| Clone ID | N/A | ||
| Concentration | 1 mg/mL | ||
| Storage buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. | ||
| Formulation | Liquid | ||
| Purification | This antibody is immunogen affinity purified. | ||
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. | ||
| Reactivity life | 12 months when stored properly according to instructions (provided antibody remains sterile). | ||
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